| Congresso Brasileiro de Microbiologia 2023 | Resumo: 1124-1 | ||||
Resumo:The current increase in the prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP) in nosocomial infections, due to the presence of genes that encode carbapenemases, represents a major public health problem as it reduces therapeutic options and increases the mortality rate. One of the main ways of environmental dissemination of this pathogen is through wastewater from wastewater treatment plants (WWTPs). The main of this study were to isolate CRKP carrying genes that encode carbapenemases from untreated (UWW) and treated (TWW) wastewater and investigate the clonal relationship. Eighteen K. pneumoniae strains recovered from three WWTPs: WWTP1 (municipal), WWTP2 (mixed/industrial) and WWTP3 (hospital) were identified by MALDI-TOF. The antimicrobial susceptibility was evaluated by disk-diffusion and the carbapenemases production by Carbapenembac® (PROBAC, BRASIL). The carbapenemases genes blaKPC, blaNDM, blaOXA-48-like, blaIMP, blaVIM, blaGES and blaBKC were investigated by real-time PCR and the clonal relationship through multi-locus sequence typing (MLST). Thirty nine percent (7/18) of isolates were classified as multidrug-resistant (MDR), 61.1% (11/18) extensively drug-resistant (XDR) and 83.3% (15/18) showed carbapenemase activity. Three carbapenemase-encoding genes were found, blaKPC (55%), blaNDM (27.8%) and blaOXA-370 (11.1%), other genes were not detected. Five different Sequence Types (STs) were identified: ST11, ST37, ST147, ST244 and ST281. ST11 and ST244, sharing four alleles were grouped into clonal complex 11 (CC11). CRKP isolates of ST11 were recovered from the UWW and TWW of WWTP1. To our knowledge, this is the first report of KPC, NDM, OXA-370 -producing ST244 in wastewater. Our results show the importance of monitoring antimicrobial resistance in WWTPs effluents to minimize the risk of spreading bacterial load and ARGs in aquatic ecosystems, using advanced treatment technologies to reduce these emerging pollutants at WWTPs. Furthermore, the occurrence and persistence of CRKP ST11 carrying different resistance genetic profiles in treated wastewater may generating negative impacts on public and environmental health. Palavras-chave: Klebsiella pneumoniae, wastewater, resistance genes, carbapenemase, MLST Agência de fomento:FAPERJ e CNPQ | |||||